Table 1 Primer sequences used for qPCR amplification
Gene | GenBank ID | Forword primer 5′–3′ | Reverse primer 5′–3′ |
|---|---|---|---|
Rat_ NppA (ANP) | NM_012612.2 | GGAAGTCAACCCGTCTCAGA | TGGGCTCCAATCCTGTCAAT |
Rat_ NppB (BNP) | NM_031545.1 | CCAGAACAATCCACGATGCA | GCAGCTTGAACTATGTGCCA |
Rat_ 18Â s rRNA | NR_046237.2 | CATTCGAACGTCTGCCCTAT | GTTTCTCAGGCTCCCTCTCC |
Human_ NPPA (ANP) | NM_006172.4 | CAGCAAGCAGTGGATTGCTCCT | TCTGCGTTGGACACGGCATTGT |
Human_ NPPB (BNP) | NM_002521.3 | GCTGCTTTGGGAGGAAGATG | ATGAGTCACTTCAAAGGCGG |
Human_ 18Â s rRNA | M10098.1 | CCCACTCCTCCACCTTTGAC | TGTTGCTGTAGCCAAATTCGTT |