Fig. 7

Characterization of TME patterns in the TIDE subtypes based on single-cell RNA-seq dataset. A Uniform Manifold Approximation and Projection (UMAP) plot was used to analyze the single-cell RNA-seq dataset. Each color represents one of the 13 cell types in Salomé’s dataset. B Heatmap showing the expression levels of TIDE marker genes in 13 cell types. C Proportions of 13 cell types in TIDE subtypes. D Terminally exhausted signals of two subtype cells assessed by weighted kernel density estimation. Red circle marks CD8Ts. E, F Differentiation trajectory of CD8⁺ T cells (CD8Ts) in BC, with a color code for pseudotime (E) and TIDE subtypes (F). (G) Expression levels of terminally exhausted signature along the CD8Ts differentiation trajectory in BC