Fig. 5

Validation of the TIDE subtypes using the collected real-world BC samples. A Consensus clustering by the TIDE marker genes can distinguish the real-world BC samples into three TIDE subtypes. B Levels and trends of TIDE scores among the TIDE subtypes. C Multi-class ROC curve analysis was used to evaluate the subtyping consistency of repeated samples. D–F The proportion of the tumor grade (D), pT (E) and pathological diagnosis (F) among the TIDE subtypes. G Typical CT tomographic images of bladder tumors ( →) and tumor volumes based on CT tomography in three TIDE subtypes. H Transcription levels of representative BC proliferation marker MKI67 in TCGA-BLCA and LY Dataset among three TIDE subtypes. I MKi67 IHC images from the collected BC samples and analysis of MKi67 positive cells among the TIDE subtypes. Scale bar, 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001. ns, no significance