Fig. 4
From: AZGP1 deficiency promotes angiogenesis in prostate cancer
Overexpression of AZGP1 inhibits angiogenesis in PC3 and DU145 tumors. A Representative images of PC3 vector control and PC3-AZGP1-OV tumor xenografts. B Calculated tumor volume, and C Tumor weight of PC3 vector control and PC3-AZGP1-OV tumor xenografts. D Quantification of the degree of redness of PC3 vector control and PC3-AZGP1-OV tumor xenografts using python, showing reduced redness in AZGP1-OV PC3 tumors. E CD31 staining showing lower microvessel density in AZGP1-OV PC3 tumors compared to controls. Microvessels were counted from 10 randomly selected high powered microscope fields uon the CD31-stained sections. F Microscopic images showing similar tumor morphology by H & E, validation of AZGP1 overexpression, and decreased number of CD31 staining microvessels in PC3-AZGP1-OV tumors compared to controls. G Representative images of DU145 vector control and DU145-AZGP1-OV tumor xenografts. H Calculated tumor volume, and I tumor weight of control and AZGP1-OV DU145 tumors. J Quantification of tumor redness using Image J, indicating reduced redness in AZGP1-OV DU145 tumors compared to vector control. K DU145 AZGP1-OV tumors displayed lower microvessel density compared to controls. L Microscopic images showing similar tumor morphology by H and E, validation of AZGP1 overexpression, and decreased number of CD31 staining microvessels in DU145-AZGP1-OV tumors compared to controls