Fig. 3

Loss of AZGP1 promotes stromal cell hyperplasia in the prostate of AZGP1^−/− mice. A Schematic representation of endogenous AZGP1, AZGP1 knockout, and targeting construct. B Genotype analysis of AZGP1 of wild-type, heterozygous AZGP1^± and homozygous AZGP1^−/− mice. C Immunofluorescence staining of CD31 and its corresponding quantification of microvessle counts per area in prostate excluding SV from wild-type and homozygous AZGP1^−/− mice. Increased microvessel density was observed in homozygous AZGP1^−/− mouse prostates. Microvessels were counted from 10 randomly selected 20X microscope fields in the CD31-stained sections. D Hematoxylin and eosin (H&E) staining of the LP lateral prostate, DP dorsal prostate and basal part of SV seminal vesicles from wild-type and homozygous AZGP1^−/− mice suggest that there are no morphological changes in LP and DP. Increased fibrosis were observed at the base of the seminal vesicles as illustrated with sirius red staining