Fig. 5

Knockdown of IL-33 promoted the PPAR signaling pathway. Following the induction of adipogenesis on day 10, 3T3-L1 cells with and without IL-33 knockdown were collected, and subjected to transcriptome profiling. (a) A scatterplot depicting the differential gene expression (DEG) values between the control cells and the IL-33-knockdown cells(fold change ≥ 1 and P ≤ 0.05). (b) The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was performed on all differentially expressed genes (DEGs). (c) A heat map illustrating DEGs between the control cells and the cells with IL-33 knockdown. (d) Real-time qPCR analyses to validate the change in gene expression revealed by RNA sequence. The data are represented as mean ± SEM of three different samples (n = 3) from at least three independent experiments. ^*P < 0.05 and ^****P < 0.0001. PPAR-γ, Peroxisome proliferator-activated receptor gamma; FABP4, Fatty acid binding protein 4; LPL, lipoprotein lipase; Adipoq, adiponectin; CD36, platelet glycoprotein 4