Fig. 2

Levels of IL-33 increased during adipogenesis. 3T3-L1 cells were induced by conventionally adipogenic differentiation and OA-induced hyperadipogenic differentiation. (a) Secretion levels of IL-33 during adipogenesis of 3T3-L1 cells in conventional and hyperadipogenic groups were measured by ELISA. (b) RT-qPCR was conducted to evaluate the mRNA level of IL-33 following adipogenesis on days 0, 2, 4, 6, 8, and 10. (c and d) Protein level of IL-33 was measured by Western blot analysis. The data are represented as the mean ± SEM (n = 3). ^**P < 0.01, ^***P < 0.001, and ^****P < 0.0001 versus the conventionally induced group at the same time points; ^#P < 0.05 and ^####P < 0.0001 versus day 0 in the conventionally induced group; ^cP< 0.001 and ^dP< 0.0001 versus day 0 in the OA-induced hyperadipogenic differentiation group