Fig. 3

Transcriptional changes of HSPCs in MDS. (A) Functional enrichment bar chart (GO terms) of DEGs in total HSPCs of MDS/sAML patients compared with HCs.|LogFC|≥1, adjusted p value < 0.05 were used to define DEGs. Adjusted p value < 0.05 was considered statistically significant for GO enrichment analysis. (B) Functional enrichment bar chart (KEGG terms) of DEGs in total HSPCs of MDS/sAML patients compared with HCs. Adjusted p value < 0.05 was considered statistically significant for KEGG enrichment analysis. (C) DEGs analysis showing up-(red) and down-(blue) regulated genes in MDS/sAML across the 11 HSPC clusters. Adjusted p value < 0.05 were used to define DEGs. (D) Dot plot of enriched terms after performing gene set enrichment analysis (GSEA) for each identified cluster. Dot color represents the enriched group, size indicates the NES absolute value, and transparency indicates the p-value. (E) Heatmap of top 60 differentially expressed genes in CMP of MDS/sAML patients compared with HCs.|LogFC|≥1 and adjusted p value < 0.05 were used to define DEGs. (F) Dot plot of enriched upregulated and downregulated KEGG terms in CMP of MDS/sAML patients. Adjusted p value < 0.05 was considered statistically significant for KEGG enrichment analysis