Fig. 2

Trajectory inference of the hematopoietic lineages and dynamic gene expression patterns of HSPCs. (A) UMAP of human BM cells. Arrows indicate main directions of differentiation, inferred from analysis of typical marker genes. (B) Reconstructed principal component graph of cell differentiation trajectory of HSPCs, colored by subpopulation identities. The upper-right trajectory plot in the square indicates the direction of pseudotime. (C) Pseudotime-ordered analysis of HSPCs from the MDS/sAML and HC samples. (D) Heatmap of gene expression dynamic trends for genes along the pseudotime in MDS/sAML patients and HCs (cataloged hierarchically into three gene modules). P value < 0.05 was considered statistically significant for Gene Ontology (GO) enrichment analysis. (E) Loess-smoothed curves fitted to the z scored averaged expression of genes in modules 1–3 along the pseudotime trajectory. (F) Two-dimensional plots showing the dynamic expression of significantly enhanced genes in MDS/sAML patients compared with HCs along the pseudotime in module 3.|LogFC|≥1 and p value < 0.05 were used to define differentially expressed genes (DEGs)