Fig. 1

The PRNP gene is a target of Wnt-β-catenin signaling in colon cancer models. A–C Analysis of the GSE200908 (A) GSE208372 (B) and GSE167008 (C) datasets reveals increased Prnp expression in cellular, organoid and in vivo mouse models of Apc inactivation. D Analysis of the GSE156083 ChIPseq dataset reveals enrichment of β-catenin and the H3K4me3 active histone mark at the promoter of the PRNP gene in SW480 and DLD-1 human colon cancer cell lines. E Analysis from the ENCODE database reveals binding of the TCF7L2-encoded TCF4 factor, together with the H3K27ac and H3K4me3 active histone marks at the promoter of the PRNP gene in the HCT116 human colon cancer cell line. F Predicted TCF7L2 binding site within the human PRNP gene promoter. G, H Relative mRNA levels of CTNNB1 (G) and PRNP (H) in CTNNB1-silenced versus control MDST8 cells, as determined in qPCR analysis. Results are expressed as means of n = 2 independent triplicates of cell preparations ± s.e.m. (***p < 0.001, **p < 0.01, Student’s t-test)