Fig. 8

Quantification of their protein levels and electrophysiological characterization in hiPSC-derived cardiomyocytes treated with small interfering ZNF528 (si-ZNF528). A Detection of target proteins in iPSC-CMs of the three groups (Mutation n = 3, CRISPR n = 3, Control n = 3) treated with small interfering ZNF528. B, C Quantitative protein analysis were performed after intervention with si-ZNF528 and blank control plasmid (si-NC) in the CRISPR group (CRISPR n = 3, CRISPR + siNC n = 3, CRISPR + siZNF528 n = 3). D The action potential-related values of the CMs in each group were compared (CRISPR n = 5, CRISPR + siZNF528 n = 5). E The potassium ion channel Kir2.1 current trace and I-V curve in a voltage-clamp pattern resulting from a holding potential of − 60 mV and test pulses ranging from -60 to + 60 mV (CRISPR n = 5, CRISPR + siZNF528 n = 5). β-Actin was used as a loading control. Bands were quantified with Image J software. One asterisk indicates p < 0.05, and two asterisks indicates p < 0.001 compared with the other groups. Data are shown as mean ± SD.*p < 0.05; data were compared by two-sample t-test