Fig. 4

Differentiation stage-related modules identified by WGCNA and motif analysis. A Heatmap of gene expression profiles clustered by WGCNA. Six homogeneity aggregation modules with the same changes in expression over time in the cardiomyocyte differentiation stage were screened (Mutation n = 6, CRISPR n = 6). B PCA of the transcriptomes derived from the mutation and CRISPR groups during cardiomyocyte differentiation. C Expression heatmap for the tan module, which shows expression heterogeneity over time (Mutation n = 6, CRISPR n = 6). D The heatmap with low expression in the mutation group was explored for subsequent alignment to matched transcription factors (Mutation n = 6, CRISPR n = 6). E The transcription factor ZNF528 was obtained after alignment, and corresponded to the duration over which chromatin accessibility was high by verifying based on ATAC-seq data obtained in this study (Mutation n = 6, CRISPR n = 6)