Fig. 2

Identification of hiPSC-derived CMs at different stages of differentiation in the CRISPR and mutation groups. A Schematic showing the experimental design used for cardiomyocyte differentiation and sample collection. Cells at different stages of differentiation were collected on days 0, 2, 4, 8, 15 and 30 for RNA-seq and ATAC-seq (Mutation n = 12, CRISPR n = 12). B Morphology of the cells at different stages of differentiation. On the 8th day, cardiomyocytes with local spontaneous beating appeared. C Immunostaining of cells with specific markers corresponding to each time stage, including TRA-1-160 (green) and OCT4 (red) for hiPSCs (day 0), Brachyury (red) for the mesoderm (day 2), α-SMA (green) and NKX2-5 (green) for days 8 and 15, and NKX2-5 (red) and TNNT2 (green) for cardiomyocytes (day 30). The nuclei were stained with DAPI (blue)