Fig. 7

Treatment with a mixture of seven short-chain fatty acid-producing bacterial strains (7-mix) and the resulting culture supernatant mixture (mix-sup) inhibits JAK/STAT3/FOXO3 pathway activation in macrophages. A-B. qRT-PCR analysis of the effect of 7-mix and mix-sup on the regulation of the JAK/STAT3/FOXO3 axis in colitic mice treated or not with clodronate (CLD) liposomes. C. Effect of 7-mix and mix-sup on the immunohistochemical expression of STAT3 and FOXO3 in colitic mice treated with CLD. D. Flow cytometry analysis of the effect of S3I-201 and fedratinib on macrophage polarization. E. qRT-PCR analysis of the effect of 7-mix, mix-sup, S3I-201, and fedratinib on the mRNA expression of JAK2, STAT3, and FOXO3 in LPS-treated RAW264.7 cells. F. Western blot analysis of the effect of 7-mix, mix-sup, S3I-201, and fedratinib on the protein expression of JAK2, p-JAK2, STAT3, p-STAT3, and FOXO3 in LPS-treated RAW264.7 cells. N = 3. *P < 0.05, **P < 0.01, ***P < 0.001