Fig. 5
Hypofractionated radiotherapy reprograms CCL8high macrophages through the CCL signaling pathway. A Volcano plot showing differentially expressed genes of the Mac_Ccl8 between the NT and RT groups. Adjusted p value < 0.05, two-sided Wilcoxon test. B Violin plots comparing the expression of Ccl2, Ccl3, Ccl4, Ccl7, Ccl8, and Ccl12 in the NT and RT groups. Unpaired two-sided Wilcoxon test. C Representative examples of multiplex immunofluorescent labeling CD206 and CCL8. Green, CD206; Red, CCL8; Blue, DAPI. D Bar plots showing the GO enrichment analysis of upregulation and downregulation genes in the RT group. E Differences in IFN-Gamma and TNF pathways activity between two groups inferred by GSEA. F Cell–cell communication network between myeloid populations and LLC cells. G River plot displaying communication patterns of different cell types. H Chord plot (top) and heatmap (bottom) showing communication network of CCL signaling pathway in different cell types. I Weighted network analysis of differential interaction strength of signals in the Mac_Ccl8 population between two groups. J The comparison of cellular communication probability from Mac_Ccl8 to other myeloid populations between two groups. K Chord plot displaying the upregulated signaling pathways in the RT group relative to the NT group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant