Fig. 2

The alteration of LLC cell states following hypofractionated radiotherapy. A UMAP plot showing clusters of LLC cells. B UMAP plot showing the distribution of LLC cells in each group. C The proportion of each cluster in control (NT) and radiotherapy (RT) groups. D Violin plot showing stemness score for each cluster. E UMAP plot and F Bar plot of stemness in LLC cells predicted by CytoTRACE. G Heatmap of hallmark gene sets from the MSigDB enriched in different types of cell clusters. H Heatmap of transcription factors activity in each cell cluster. I Dot plot showing outgoing communication signaling pathways of different cell types. J Chord plot of communication network of CCL signaling pathway between tumor cells and other cell types. K Csf1 gene expression level between NT and RT groups. L The relative mRNA expression levels of Csf1 in LLC cells with or without exposure to 8 Gy of radiation in vitro. Data are representative of three independent experiments