Fig. 1

Single-cell transcriptomic landscape of subcutaneous LLC tumors at early-stage following hypofractionated radiotherapy. A Overview of the experimental design for single-cell RNA sequencing. B Uniform Manifold Approximation (UMAP) plot showing the unsupervised clusters of 54883 single cells and annotated cell types. C The proportion of each cell types in LLC tumors treated with or without radiation. For B and C, each color represents the same cell type. D Feature plot and E Dot plot showing marker genes. F Heatmap showing serum chemokines level in LLC murine models 72 h post-treatment. Each row in the heatmap has been scaled. G The concentrations of CCL2, CCL7, CCL8, and CSF1 in serum measured by ELISA (n = 5/group). Data are presented as mean ± SD with student unpaired t test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant