Fig. 6

OCT1 binds to the FSTL1 promoter. (A) Peak map of OCT1 binding to the FSTL1 promoter. (B–C) OCT1 ChIP-agarose gel and qPCR showing OCT1 binding to the FSTL1 promoter. (D) Putative OCT1 motifs in the FSTL1 promoter by JASPAR. (E) Wild-type and mutant FSTL1 promoter sequences. (F) OCT1 overexpression verification by western blot. (G–H) Dual-luciferase reporter assay analyzing OCT1 binding to the FSTL1 promoter. (I) ChIP-agarose gel showing OCT1 binding to the FSTL1 promoter with OCT1 overexpression versus control. (J) Western blot of EMT markers after OCT1 knockdown. (K) Western blot of EMT markers after OCT1 overexpression rescue of FSTL1 knockdown. (L–N) Colony formation after OCT1 overexpression rescue, scale bars 1 cm. (O–R) Migration by scratch assay after OCT1 overexpression rescue, scale bars 50 μm. Data were presented as the mean ± SD. (ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)