Fig. 3
FSTL1 promotes proliferation and invasion in vitro and in vivo. (A–B) FSTL1 knockdown efficiency by western blot. (C–D) FSTL1 overexpression efficiency by western blot. (E–F) Clone formation after FSTL1 knockdown, scale bars 1 cm. (G–H) Clone formation after FSTL1 overexpression, scale bars 1 cm. (I–J) Cell viability by CCK8 assay. (K–L) Invasion by Transwell after FSTL1 knockdown, scale bars 200 μm. (M–N) Invasion by Transwell after FSTL1 overexpression, scale bars 300 μm. (O–P) Migration by scratch assay after FSTL1 knockdown, scale bars 50 μm. (Q–R) Migration by scratch assay after FSTL1 overexpression, scale bars 200 μm. (S–T) Tumor size, weight and volume. (U) IHC for Ki67, FSTL1, E-cadherin and N-cadherin, scale bars 50 μm. Data were presented as the mean ± SD. (ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)