Fig. 1

FSTL1 expression is upregulated in cSCC. (A) Venn diagram of differentially expressed genes in GSE32628 and GSE66359. (B-C) Heatmaps of differentially expressed gene expression in GSE32628 and GSE66359. (D) FSTL1-FSTL5 fold changes in the datasets. (E) FSTL1 mRNA expression in the datasets. (F) FSTL1 western blot of tissues, NC - normal control, T - tumor. (G) FSTL1 western blot of cell lines. (H) H&E staining of tissues, scale bars 200 μm. (I) FSTL1 immunohistochemistry of tissues, scale bars 200 μm. (J) FSTL1 immunocytochemistry in A431 cells, scale bars 50 μm. Data were presented as the mean ± SD. (ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)