Fig. 7

TNFSF11 overexpressing cells are more susceptible to ferroptosis inducers. (A-D) Cells were treated with various concentrations of RSL3 and erastin for 72 h and cell viability was measured using CCK8 assays. Statistical significance levels were shown above the curves. Bars, means ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, NS: no significant. (E-H) Cells were treated with RSL3, erastin or ferrostatin-1 for 24 h or 48 h, and subjected to CCK8 assay. Bars, means ± SD, n = 5. **P < 0.01, ***P < 0.001, NS: non-significant. (I-L) Intracellular ROS levels were evaluated using flow cytometry in cells post treatment with DMSO, erastin, or co-incubation with erastin and ferrostatin-1 for 4 h. MitoSox Red is used as a dye to detect ROS. All results were repeated for three times. Fer: Ferrostatin-1