Fig. 4

Propofol inhibits Rab5a expression and the interaction between Rab5a and TLR4 in macrophages. BMDMs were treated with LPS (1 μg/ml) for the indicated time with or without pretreatment of propofol (50 μM) for 30 min. A Western blotting of Rab5a in BMDMs. B Interaction of TLR4 withRab5a determined by Co-IP analyses in BMDMs. Immunoprecipitation (IP) and immunoblotting (IB) were performed with anti-TLR4 and anti-Rab5a antibodies. Data are expressed as the mean ± SD, n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, 1-way ANOVA with Tukey’s post hoc test