Fig. 3

Knockout of Rab5a attenuates membrane TLR4 expression on macrophages. Wild type and Rab5a^−/− mice that underwent CLP were sacrificed after 12 h. A Membrane TLR4 expression of peritoneal macrophages from CLP mice was detected by flow cytometry. B Serum proinflammatory factor levers of CLP mice were assessed by ELISA. C The survival curve of Wild type and Rab5a^−/− mice underwent CLP. Kaplan Meier analysis was used to evaluate the survival rate of CLP mice (n = 15). D H&E staining and histological score of lung, liver, kidney, and intestinal in wild type and RAB5a^−/− mice, Scale bars: 50 μm. Data are expressed as the mean ± SD, n = 8, *p < 0.05, **p < 0.01, ***p < 0.001, unpaired t test or 1-way ANOVA with Tukey’s post hoc test