Fig. 1

Propofol inhibits membrane TLR4 expression of macrophages after LPS stimulation or CLP. BMDMs were treated with LPS (1 μg/ml) for the indicated time with or without pretreatment of propofol (50 μM) for 30 min. C57BL mice that underwent CLP were pretreated with propofol (50 mg/kg) or equal volume of fat emulsion for 30 min and sacrificed after 12 h. A Proinflammatory factor mRNA levels in BMDMs were measured by qPCR. B Serum proinflammatory factor levers of CLP mice were assessed by ELISA. C Membrane TLR4 expression of BMDMs was analyzed by flow cytometry. D Western blot analysis of membrane TLR4 expression of BMDMs. E Membrane TLR4 expression of peritoneal macrophages from CLP mice was detected by flow cytometry. Data are expressed as the mean ± SD, n = 5–8, *p < 0.05, **p < 0.01, ***p < 0.001, 1-way ANOVA with Tukey’s post hoc test. PPF propofol