Fig. 7
Antitumor effect and safety evaluation of the chemoimmunotherapy hydrogel. A Treatment schema of R837/Nab-PTX/ALG in tumor suppression experiment. 1 × 106 murine breast cancer cells 4T1 in 100 µL PBS were injected subcutaneously on the lower right mammary gland of Balb/c mice to establish 4T1 tumor mouse models. B Average tumor-growth curves of Balb/c mice bearing 4T1 breast cancer with different treatments as indicated (n = 6). The mice were administered with R837 (50 µg) and/or Nab-PTX (100 µg) on day 6 and day 13, which were dissolved in normal saline or sodium ALG solution to a final volume of 100 µL per dose. The tumor size was measured every 2–3 days from the first day of administration until the average volume of the NS group reached 1000 mm3, which was considered to have reached the standard for euthanasia of mice. The error bars represented mean ± SEM and p-values were calculated by two-way ANOVA and Tukey post-test and correction. ***P = 0.0005, ****P < 0.0001. C Survival curves of Balb/c mice in different groups (n = 6). P-values were calculated by log-rank (Mantel–Cox) test, *P < 0.0194, **P < 0.0045, ***P = 0.0006 (NS vs R837/ALG, NS vs Nab-PTX/ALG, NS vs R837/Nab-PTX/ALG). D Hematoxylin–eosin staining of main organs, including heart, liver, spleen, lung and kidney. (the scale bar is 200 µm)