Fig. 3

In vitro function of the chemoimmunotherapy hydrogel. A Representative flow cytometry images of mature DCs (mDCs, CD11c⁺CD80⁺CD86⁺) after co-incubation with R837 (5 µg mL⁻¹), normal saline (NS, negative control), or LPS (1 µg mL⁻¹, positive control) in vitro for 48 h. B The percentage of mDCs after co-incubation with R837, NS or LPS in vitro for 48 h (n = 3). The error bars represented mean ± SEM. P-values were calculated by two-tailed unpaired Student’s t-tests. ***P = 0.0004 (R837 vs NS) 0.0001 (LPS vs NS). C Cumulative release of R837 and nanoparticle albumin-bound-paclitaxel (Nab-PTX) from sodium alginate solution (ALG) based hydrogel in vitro at 37 ℃. D Cell viability of human renal epithelial cells 293 T after co-incubation with NS, Nab-PTX (10 mg mL⁻¹), or ALG loaded with Nab-PTX (Nab-PTX/ALG) in vitro for 24 h, 48 h or 72 h respectively (n = 5). The error bars represented mean ± SEM. p-values were calculated by two-tailed unpaired Student’s t-tests. ***P = 0.0005, ****P < 0.0001. E Cell viability of mouse breast cancer cells 4T1 after co-incubation with NS, Nab-PTX (10 mg mL⁻.¹), or ALG loaded with Nab-PTX (Nab-PTX/ALG) in vitro for 24 h, 48 h or 72 h respectively (n = 5). The error bars represented mean ± SEM. p-values were calculated by two-tailed unpaired Student’s t-tests. *P = 0.0312, **P = 0.004, ***P = 0.0017