Fig. 5

Ablation of MAD2L2 suppresses BC cell progression via p53 DDR pathway. A, B Cell growth was detected by CCK-8 assay (A) and colony-forming assay (B). C KI67 detection using IF staining. D Cell cycle analysis was measured by flow cytometry. E Representative images of SA-β-gal staining. F Western blot analysis of MAD2L2, CyclinD1, p53, p21 and γH2A.X expression. G The wound healing assay for T24 and 5637 cells. H The transwell assay for T24 and 5637 cells. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)