Fig. 4

AURKB ablation upregulates the p53 pathway and suppresses BC cell progression via MAD2L2. A-B Cell growth was detected by CCK-8 assay (A) and colony-forming assay (B). C KI67 detection using IF staining. D Cell cycle analysis was measured by flow cytometry. E Representative images of SA-β-gal staining. F Western blot analysis of AURKB, MAD2L2, CyclinD1, p53, p21 and γH2A.X expression. G The wound healing assay demonstrated the capacity of migration. H The transwell assay demonstrated the capacity of invasion. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)