Fig. 3

AURKB interacts with and promotes the expression of MAD2L2. A Protein interaction network map of proteins bound to AURKB. B GO/KEGG enrichment analysis of AURKB in TCGA database. C GSEA results of AURKB in TCGA database. D Western blot analysis of AURKB, MAD2L2, CyclinD1, p53, p21 and γH2A.X expression. E Co-IP indicated the interaction between AURKB and MAD2L2 in BC cells. F T24 and 5637 cells were subjected to IF staining for AURKB (red) or MAD2L2 (green) and nuclei with DAPI (blue). G Representative IHC images of MAD2L2 in BC tissues and adjacent normal tissues. H The correlation analysis of AURKB and MAD2L2 H-score of IHC in patient samples. I The correlation analysis between AURKB and MAD2L2 expression in TCGA database. J Differential MAD2L2 expression in BC tissues and normal bladder tissues in unpaired and paired samples. K Kaplan–Meier survival curves for OS and DSS comparing the high and low expression of MAD2L2 in TCGA database. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)