Fig. 8

ER stress induced by inhibition of HTRA1 activated ER autophagy. ER stress indicators like GRP78, ATF6, p-EIF2A and p-IRE1 western blotting and quantitative data in cardiac fibroblasts transfected with HTRA1 siRNA (A) or HTRA1 plasmid (B). Grey represented the control group, blue represented the HTRA1 siRNA group, and red represented the HTRA1 plasma group. Representative western blotting (C) and quantitative data (D) showing the effect of broad ER stress inhibitor tauroursodeoxycholic acid (TUDCA, 0.5 mmol/l, 24 h) on ER autophagy in cardiac fibroblasts transfected with HTRA1 siRNA. GRP78, Beclin1 and LC3B were detected. (E). Live-cardiac fibroblasts imaging in different groups transfected with the ssRFP-GFP-KDEL tandem reporter plasmid for 24 h. The scale bar indicates 2 μm