Fig. 3

Targeted inhibition of HTRA1 can improve Dox-induced myocardial fibrosis and cardiac contractile dysfunction. A Animal experiment process, including AAV9 injection and Dox intervention. B Immunofluorescence staining of tissues showing the infection efficiency of AAV9 in mouse heart. The lower scale bar indicates 500um, and the higher scale bar indicates 20 μm. Representative echocardiography images (C) and quantitative data (D) assessing the difference of LVEDd and EF% in different groups (n = 6). Masson staining (E) and quantitative data (F) showing the fibrosis abundance in DCM mice with or without HTRA1 inhibition. The lower scale bar indicates 500um, and the higher scale bar indicates 20 μm. Fibrogenic proteins including col1, α-SMA, CTGF and Fib western blotting (G) and quantitative data (H) in DCM mice with or without HTRA1 inhibition