Fig. 4

Characterizing a murine syngeneic brainstem DMG model. a Intracranial implantation of an H3K27M mutant DMG cell line was achieved by creating a burr hole in the skull at a location posterior to the lambda and lateral to the sagittal suture, with cells injected at a depth of 5.5 mm. b Representative images of non-contrast T2-weighted MRI of tumor-bearing mice. c Representative photomicrographs of H&E-stained tissue from tumor-bearing mice. d Representative H&E staining of normal parenchyma, tumor core, and peritumoral region showing the histopathologic features of murine DMG xenograft. Arrows indicate microhemorrhage