Fig. 4

MiR-217-5p in exosomes from senescent epithelial cells mediates fibroblasts activation. A qPCR analysis of the miRNAs expression in MLE-12 cells treated with or without PQ. B qPCR analysis of the miRNAs expression in the exosomes isolated from MLE-12 cells treated with or without PQ. C qPCR analysis of miR-217-5p expression in pulmonary fibroblasts co-cultured with MLE-12 cells or PQ-treated MLE-12 cells. D qPCR analysis of miR-217-5p expression in the lung tissues from mice treated with or without PQ. E qPCR analysis of the expression of miR-217-5p, Acta2 and Col1a1 in pulmonary fibroblasts transfected with or without miR-217-5p mimic. F Western blot analysis of the expression of α-SMA and Collagen I in pulmonary fibroblasts transfected with or without miR-217-5p mimic. G Western blot analysis of the expression of α-SMA and Collagen I in pulmonary fibroblasts co-cultured with PQ-treated MLE-12 cells transfected with or without miR-217-5p inhibitor. n = 3 per group. All data were presented as the means ± SEM. *P < 0.05