Fig. 3

Senescent epithelial cell-derived Exosomes promote the activation of fibroblast. A, B The mRNA and protein levels of α-SMA and Collagen I in pulmonary fibroblasts treated with the exosomes isolated from MLE-12 cells (MLE-12 Exo) or PQ-treated MLE-12 cells (PQ-MLE-12 Exo). C The EdU assay of pulmonary fibroblasts treated with MLE-12 Exo or PQ-MLE-12 Exo. D The representative H&E staining (up panel) and Masson’s trichrome staining (down panel) images of the lung tissues from mice intratracheally injected with MLE-12 Exo or PQ-MLE-12 Exo. n = 5 per group. E, F Western blot and immunofluorescent analysis of the expression of α-SMA (Green) and Collagen I (Red) in the lung tissues from mice intratracheally injected with MLE-12 Exo or PQ-MLE-12 Exo. All data were presented as the means ± SEM. *P < 0.05