Fig. 2

Senescent epithelial cells induce fibroblast activation through exosome secretion. A qPCR analysis of the mRNA levels of Acta2 and Col1a1 in pulmonary fibroblasts co-cultured with GW4869-pretreated MLE-12 cells or senescent MLE-12 cells (Sn-MLE-12). B Western blot analysis of the expression of α-SMA and Collagen I in pulmonary fibroblasts co-cultured with GW4869-pretreated MLE-12 cells or Sn-MLE-12 cells. C Concentration of exosomes from BALF of PQ-poisoned mice injected with or without GW4869. n = 5 per group. D, E qPCR and Western blot analysis of the mRNA (D) and protein (E) levels of α-SMA and Collagen I in the lung tissues from PQ-poisoned mice injected with or without GW4869. All data were presented as the means ± SEM. *P < 0.05