Fig. 1
PQ promotes epithelial cell senescence and exosome secretion in vivo and in vitro. A Representative images and quantification of coimmunostaining of SPC (Green) and p16INK4a (Red) in the lung tissues from the mice treated with or without PQ. n = 5 per group. B The representative H&E staining (up panel) and Mssson’s trichrome staining (down panel) images of the lung tissues from mice intratracheally injected with MLE-12 cells or senescent MLE-12 cells (Sn-MLE-12). n = 5 per group. C Western blot analysis of the expression of α-SMA and Collagen I in the lung tissues from mice intratracheally injected with MLE-12 cells or Sn-MLE-12 cells. D The representative H&E staining (up panel) and Masson’s trichrome staining (down panel) images of the lung tissues from PQ-poisoned mice treated with or without ABT263. n = 5 per group. E Western blot analysis of the expression of α-SMA and Collagen I in the lung tissues from PQ-poisoned mice treated with or without ABT263. F Concentration of exosomes from BALF of mice treated with or without PQ. n = 5 per group. G The concentration of exosomes in the supernatant of MLE-12 cells treated with or without PQ. n = 3 per group. All data were presented as the means ± SEM. *P < 0.05