Fig. 6

THOC5 promotes gallbladder cancer metastasis through lipid metabolism. A, B Transwell assays of NOZ and ZJU-0430 cells transfected with siRNAs and plasmids. Scale bars, 100 μm. C, D Lipid droplets in NOZ cells after THOC5 overexpressing and knockdown were stained by BODYPI. E Total acylcarnitine in NOZ cells in indicated cells. F Protein levels of CPT2 and p-JNK were determined in NOZ cells after incubating with different concentrations of acylcarnitine for 6 h. G Protein levels of CPT2 and p-JNK in NOZ cells after incubating with 5 μM acylcarnitine for different hours. H, I Protein levels of CPT2 and p-JNK in GBC cells after THOC5 knockdown or overexpressing. J Protein levels of CPT2 and p-JNK in NOZ cells in indicated cells