Fig. 4
From: Acylcarnitines promote gallbladder cancer metastasis through lncBCL2L11-THOC5-JNK axis
N6-methyladenosine modified is associated with the expression of lncBCL2L11. A Protein levels of ALKBH5 and METTL3 in NOZ cells cocultured with different concentrations of acylcarnitines. B Expression of lncBCL2L11 in NOZ cells co-cultured with acylcarnitine was detected by RT-PCR. C Expression of lncBCL2L11 was tested after METTL3 knockdown in NOZ and GBC-SD cells. D RIP assay was performed by anti-m6A antibody. LncBCL2L11 was detected by RT-PCR. E Methylation enrichment of lncBCL2L11 co-cultured with acylcarnitines was detected by m6A RIP. F Expression of lncBCL2L11 was detected by RT-PCR after actinomycin D inhibition (p=0.007)