Fig. 3

lncBCL2L11 promotes GBC cell metastasis by reducing lipid metabolism in vitro and in vivo. A Cell proliferation assay for NOZ and GBC-SD cells transfected with siRNAs were determined using CCK-8 assay. B, C Transwell assays were performed in NOZ and GBC-SD cells. Scale bars, 100 μm. D Liver metastasis in mice at 6 weeks after intrasplenic injection with NOZ, red arrows indicate metastatic nodules(left). Representative isolated liver tissue sections were stained by hematoxylin–eosin(right). Scale bars, 100 μm. E Tumor number of liver metastases was measured in mice after lncBCL2L11 lentivirus transfected. F, G Lipid droplets in NOZ and GBC-SD cells after lncBCL2L11 overexpressing and knockdown were stained by BODYPI. H Total acylcarnitines in indicated cells detected by LS/MS. I Protein levels of CPT2, JNK, p-JNK in NOZ cells after overexpressing or knockdown lncBCL2L11. J The correlation between the expression of lncBCL2L11 and CPT2 was determined through a linear regression analysis. Paired t test. K Expression of lncBCL2L11 was detected via Real-time PCR in 40 paired GBC tissues