Table 1 Antibodies information
Antibodies name #Cat. No | Source | Species | Application | Dilution rate |
|---|---|---|---|---|
Anti-iNOS antibody #ab15323 | Abcam | Rb | WB, IF | 1:250, 1:100 |
NOX2 Polyclonal antibody #19,013–1-AP | Proteintech | Rb | WB, IF | 1:1000, 1:200 |
NADPH oxidase 4/NOX4 Rabbit mAb #48,782 | SAB | Rb | WB, IF | 1:1000, 1:200 |
COX2 (D5H5) XP® Rabbit mAb #12,282 | CST | Rb | WB, IF | 1:1000, 1:200 |
SIRT1 Polyclonal antibody #13,161–1-AP | Proteintech | Rb | WB, IF | 1:1000, 1:200 |
PGC1a Monoclonal antibody #66,369–1-Ig | Proteintech | Rb | WB | 1:1000 |
DRP1 (C-terminal) Polyclonal antibody, #12,957–1-AP | Proteintech | Rb | WB, IF | 1:1000, 1:100 |
TOM20 Polyclonal antibody, #11,802–1-AP | Proteintech | Rb | IF | 1:100 |
HRP-conjugated Beta Actin Monoclonal antibody #HRP-60008 | Proteintech | Ms | WB | 1:10,000 |
Goat Anti-Rabbit IgG Secondary antibody (H + L), HRP #YFSA02 | YIFEIXUE BioTech | Goat | WB | 1:10,000 |
Goat Anti-Mouse IgG Secondary antibody (H + L), HRP # YFSA01 | YIFEIXUE BioTech | Goat | WB | 1:10,000 |
Anti-Iba1 antibody [EPR16588] #ab178846 | Abcam | Rb | IF | 1:500 |
Alexa Fluor® 594 AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H + L) #111,587,003 | Jackson ImmunoResearch | Goat | IF | 1:500 |
Alexa Fluor® 488 AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H + L) #111,547,003 | Jackson ImmunoResearch | Goat | IF | 1:500 |
Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG (H + L) #115,586,003 | Jackson ImmunoResearch | Goat | IF | 1:500 |
Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG (H + L) #115546,003 | Jackson ImmunoResearch | Goat | IF | 1:500 |