Fig. 6
The effects of CAPE after SCI were dependent on SIRT1. A WB analysis of SIRT1, PGC1α, and DRP1 levels in HMGB1-stimulated BV-2 microglial cells after CAPE, NAM and SRT2183 treatment; n = 3. β-actin was used as the control. B Bar graph showing a quantitative analysis of SIRT1 expression; n = 3. C Bar graph showing a quantitative analysis of PGC1α expression; n = 3. D Bar graph showing a quantitative analysis of DRP1 expression; n = 3. E Representative immunofluorescence labeling images for SIRT1 (red) and IBA-1 (green) within HMGB1-stimulated BV-2 microglial cells after CAPE, NAM and SRT2183 treatment; n = 3. Scale bar = 50 μm. F Representative immunofluorescence labeling images for DRP1 (red) and IBA-1 (green) within HMGB1-stimulated BV-2 microglial cells after CAPE, NAM and SRT2183 treatment; n = 3. Scale bar = 50 μm. G WB analysis of iNOS, COX-2, NOX-2, and NOX-4 levels in HMGB1-stimulated BV-2 microglial cells after CAPE, NAM and SRT2183 treatment; n = 3. β-actin was used as the control. H Bar graph showing a quantitative analysis of iNOS expression; n = 3. I Bar graph showing a quantitative analysis of COX-2 expression; n = 3. J Bar graph showing a quantitative analysis of NOX-2 expression; n = 3. K Bar graph showing a quantitative analysis of NOX-4 expression; n = 3. L Representative immunofluorescence labeling images of TOM20 (red) and IBA-1 (green) within HMGB1-stimulated BV-2 microglial cells after CAPE, NAM and SRT2183 treatment; n = 3. Scale bar = 50 μm. M Representative immunofluorescence labeling images of ROS (green) within HMGB1-stimulated BV-2 microglial cells after CAPE, NAM and SRT2183 treatment; Scale bar = 50 μm. N Microglial mitochondrial membrane potential analyzed by JC-1 staining; Scale bar = 50 μm. Data are shown as means ± SEM. Statistical significance was determined with one-way ANOVA followed by Tukey’s post hoc test. #p < 0.05 vs. Control group, *p < 0.05 vs. HMGB1 group, &p < 0.05 vs. CAPE group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. = no significance