Fig. 5

After mouse SCI, CAPE effectively regulated the SIRT1/PGC1α/DRP1 signaling axis. A WB analysis of SIRT1, PGC1α, and DRP1 levels in injured spinal cords at 7 dpi; n = 3. β-actin was used as the control. B Bar graph showing a quantitative analysis of SIRT1 expression; n = 3. C Bar graph showing a quantitative analysis of PGC1α expression; n = 3. D Bar graph showing a quantitative analysis of DRP1 expression; n = 3. E Double immunofluorescence labeling of microglia for IBA-1(green) and SIRT1 (red), obtained from longitudinal Sects. 1 mm caudal to the lesion site at 7 dpi. Scale bar = 50 μm. F Double immunofluorescence labeling of microglia for IBA-1(green) and DRP1 (red), obtained from longitudinal Sects. 1 mm caudal to the lesion site at 7 dpi. Scale bar = 50 μm. G Double immunofluorescence labeling of microglia for IBA-1(green) and TOM20 (red), obtained from longitudinal Sects. 1 mm caudal to the lesion site at 7 dpi. Scale bar = 50 μm. Data are shown as means ± SEM. Statistical significance was determined with one-way ANOVA followed by Tukey’s post hoc test. ^#p < 0.05 vs. Sham group, *p < 0.05 vs. SCI group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. = no significance