Fig. 7

LIPUS inhibits the progression of OA via YAP/RIPK1 axis. A Western blot and quantitative analysis of ACAN COL2A1, SOX9 and MMP3 expression level. B Western blot and quantitative analysis of COX2 and iNOS expression level. C Western blot of P-YAP and P-P65 expression level. D, E Immunofluorescence staining and fluorescence intensity analysis of COL2A1 and MMP13 relative expression level. F Chondrocytes were transfected with mRFP-GFP-LC3 adenovirus following IL-1β treatment for 24 h. The representative images of fluorescence and quantitative analysis of red dots and yellow dots were revealed (scale bar: 10 μm). G Western blot of P-YAP, YAP, P-P65, P65, P-IκB, IκB, P-RIPK1 and RIPK1 relative protein expression. H Images of IF staining for P65 relative expression and the distribution in chondrocytes with IL-1β induction for 15 min (scale bar: 25 μm). I Co-IP experiment of the binding between YAP and RIPK1 after LIPUS intervention. J YAP and RIPK1 colocalization in chondrocytes were detected by IF after LIPUS intervention (scale bar: 10 μm). K, L Immunohistochemistry staining to show the P-YAP-positive cell, P-RIPK1-positive cell and P-P65-positive cell in cartilage of the six groups (scale bar: 100 μm). Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001