Fig. 5

YAP regulates NF-κB signaling pathway. A, B Western blot and quantitative analysis of P-P65, P-IKKα/β and P-IκB relative protein expression with si-NC or si-YAP following IL-1β induction. C, D Western blot and quantitative analysis of P-YAP, YAP, P-P65 and P65 relative protein expression with oe-NC or oe-YAP following IL-1β induction. E, F Images of IF staining and statistical analysis for P65 relative expression and the distribution in chondrocytes transfected with si-NC or si-YAP following IL-1β induction for 15 min (scale bar: 35 μm). G Co-IP experiment of the binding between YAP and RIPK1 was shown. H YAP and P-RIPK1 colocalization in chondrocytes were detected by IF (scale bar: 10 μm). Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001