Fig. 2

Autophagy is associated with OA. A, B Immunohistochemistry images and quantitative analysis of P62-positive cells in knee joint cartilage of rats (sham group: n = 8; DMM group: n = 8, scale bar: 100 μm). C Western blot and quantitative analysis of LC3 expression level after IL-1β treatment the chondrocytes for 0, 1, 6, 12, 24 and 48 h. D Western blot and quantitative analysis of COL2A1, MMP13, COX2 and iNOS expression level. E Western blot and quantitative analysis of P62 and LC3 expression level. F Western blot and quantitative analysis of P-YAP and YAP expression level. G, H Chondrocytes were transfected with mRFP-GFP-LC3 adenovirus following IL-1β treatment for 24 h. The representative images of fluorescence and quantitative analysis of red dots (autolysosomes) and yellow dots (autophagosomes) were shown (scale bar: 10 μm). I Co-IP experiment of the binding between YAP and P62 was shown. J YAP and P62 colocalization in chondrocytes were detected by IF (scale bar: 10 μm). Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001