Fig. 7

ZEB1 is a downstream factor of HIF-1α. A CUT&RUN assay showing the relative enrichments of DNA bound to the ZEB1 promoter at binding sites treated with CAFs-CM and corresponding HIF-1α knockdown cells. B Scatter plot illustrating the correlation between ZEB1 mRNA expression and HIF-1α mRNA expression. C Western blotting detection of ZEB1 protein expression in ovarian cancer (OC) cells with knockdown of HIF-1α. D Relative expression levels of ZEB1 protein by imageJ software. E Knockdown of ZEB1 in OC cells pretreated with or without CAFs-CM, followed by western blotting analysis of HIF-1α and EMT markers expression. F Relative expression levels of HIF-1α and EMT markers by imageJ software. Results are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001