Fig. 2

Screening of prognostic-related genes derived from CAFs. A Venn diagram illustrating the overlap of 510 key genes identified in both the RNA-seq cohort and GPL570 cohort. B Gene Ontology (GO) enrichment analysis demonstrating the functional significance of the identified key genes derived from CAFs. The bubbles represent different biological processes (BP), cell localizations (CC), and molecular functions (MF), with each bubble's size corresponding to the number of enriched genes in that specific category. C Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis further validating the functional relevance of the key genes derived from CAFs. The size of each bubble represents the number of enriched genes associated with specific pathways. D The upper image (100 × magnification) shows 60–70% confluent cells, while the lower image shows purified CAFs or NFs (200 × magnification). E Western blotting analysis presenting the expression of representative markers (α-SMA, CD31, E-cadherin, and Vimentin). F RT-qPCR analysis presenting the expression of the representative marker Vimentin. G RT-qPCR analysis presenting the expression of the representative marker α-SMA. H Western blotting analysis of the expression of epithelial-mesenchymal transition (EMT) markers in different tissues. I Representative morphological images obtained through multiple fluorescence, IHC, and H&E staining techniques, depicting tissues from OC tissues. The purple color represents α-SMA positivity, the orange color indicates Vimentin positivity, and the blue color indicates DAPI staining for nuclear identification. Scale bar: 100 μm. J Western blotting analysis of the expression of EMT markers in A2780 and HO8910 cells pretreated with CAFs-CM. K IHC analysis of the expression of ACTA2 and LYVE1 in tumor tissues from vivo assays (10 × magnification). Histogram of the IHC score in different samples. CAFs positive samples (n = 5), CAFs negative sampels (n = 5). Results are presented as the mean ± SD of three independent experiments. ****P < 0.0001, ns not significant