Fig. 6

Effect of eF-DnaK and ARV-1502 on viability of HCT116 and AGS cell line treated with cisplatin and 5FU. Cisplatin 25 µM (A–C) and 5FU 75 µM (B–D) were added to each well with the indicated cell line alone or in combination with eF-DnaK. Parallel wells of untreated cells were used as negative control. Cell viability was assessed by using the trypan blue assay. Percentage of alive cells for each treatment are calculated as percentage using the untreated cell as reference. Results are representative of 3 independent experiments for each treatment. Statistical differences were tested using Student’s t test. All statistical tests were two sided. *p < 0.05, **p < 0.01, ***p < 0.001. Treatment with ARV-1502 alone showed on average 5–8% reduction in cell viability (data not shown to maintain a clearer visibility of the results)