Fig. 6

Exploring the differentiation trajectory of high-AIF-expressing cell types. A UMAP was utilized as a dimensionality reduction technique to project a reclustered Seurat clustering system of high-AIF-expressing cell types. B Bubble plot of myeloid cell subset marker genes. C Box plot of expression patterns of AIF1 in the HLA-B27-negative and HLA-B27-positive groups. D, E Pseudotime trajectory analysis showing high AIF-expressing cell types. The cells are coloured according to cell type (D) and pseudotime value (E). F The trend of AIF1 expression along pseudotime variation. G Heatmap of expression of the top 5 marker genes for three distinct subsets of dendritic cells, along with enrichment analysis. H BEAM analysis of predifferentiated nodes associated with DC fate 1. I BEAM analysis of predifferentiated nodes according to DC fate 2. BEAM, branched expression analysis modelling