Fig. 5
From: Intestinal Piezo1 aggravates intestinal barrier dysfunction during sepsis by mediating Ca2+ influx
Ca2+ Promoted TNF-α-induced intestinal barrier disruption in Caco-2 cells. A Calcium imaging of Caco-2 cell (n = 10 for each group). B, C Mitochondrial ROS were measured by MitoSOX, and quantification of immunofluorescence analysis (n = 3 for each group). Scale bar, 50 μm. D, E Mitochondrial ROS were measured by MitoSOX, and quantification of immunofluorescence analysis (n = 3 for each group). Scale bar, 50 μm. F, G Mitochondrial membrane potentia were measured by JC-1 probes, the relative IOD ratio quantitative analysis was performed using the ratio of red to green fluorescence. (n = 3 for each group). Scale bar,50 μm. H–J Immunofluorescence analysis of ZO1 and Occludin in Caco-2 cell monolayers, and quantification of immunofluorescence analysis (n = 3 for each group). Scale bar, 20 μm